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Comparative studies on highly metabolically active histone acetylation.

M Moore, V Jackson, L Sealy

    Biochimica Et Biophysica Acta
    |January 26, 1979
    PubMed
    Summary
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    Histone acetylation is a rapid process in dividing cells, independent of cell division rate and only slightly affected by actinomycin D. This phenomenon is observed in both cancerous and normal cells across different species.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Background:

    • Histone acetylation is a crucial post-translational modification involved in gene regulation.
    • Rapid hydrolysis of histone acetate was previously observed in dividing hepatoma cells.

    Purpose of the Study:

    • To further analyze histone acetate hydrolysis in hepatoma cells across different cell cycle stages.
    • To investigate the sensitivity of histone acetylation to actinomycin D and its relation to cell division rate.
    • To examine histone acetylation in diverse cell types, including normal and cancerous cells.

    Main Methods:

    • Analysis of hepatoma tissue culture cells at various cell cycle stages and in stationary phase.
    • Treatment of cells with actinomycin D.
    • Investigation in Tetrahymena pyriformis macronuclei, bovine thymocytes, and human foreskin fibroblasts.

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    Main Results:

    • Histone acetylation is a highly metabolically active process.
    • While altered in mitotic cells, histone acetylation is largely independent of the overall cell division rate.
    • Histone acetylation shows only slight sensitivity to actinomycin D.

    Conclusions:

    • The phenomenon of histone acetylation is conserved across various cell types, including cancerous and normal cells.
    • This process appears to be common to cells from different evolutionary stages.