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Updated: Aug 18, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
Published on: February 4, 2011
Fast and precise positioning of single cells on planar electrode substrates
H Thielecke1, T Stieglitz, H Beutel
1Fraunhofer Institute for Biomedical Engineering, Sensor System/Microsystems Department, St. Ingbert, Germany. Hagen.Thielecke@ibmt.fhg.de
Abstract:
For cell biosensors and for studying neural networks using planar electrode substrates, a suitable technique for positioning single cells on electrodes was needed. We reported a new method for fast and efficient positioning of single cells on ring electrodes by controlled suction through holes. We described the microfabrication of electrode substrates with microholes and the cell positioning procedure. L929 cells and Neuro 2A cells could be positioned in parallel without cell damage.
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