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Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Existence of a bioactive lipid, cyclic phosphatidic acid, bound to human serum albumin
T Kobayashi1, R Tanaka-Ishii, R Taguchi
1Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan. tetkoba@cc.ocha.ac.jp
Abstract:
A novel bioactive lipid, cyclic phosphatidic acid (cPA), was identified in lipids bound to human serum albumin. A cPA fraction was extracted and purified from human serum albumin by use of a combination of preparative TLC and HPLC. Electrospray ionization mass spectrometry of the purified fraction showed molecular ions corresponding to cPA, which was composed of some different fatty acid species. The most abundant component was identified as palmitoyl-cPA by tandem mass spectrometry using collision-induced dissociation. These data have established that cPA is a naturally occurring lipid bound to human serum albumin.
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