A lapping apparatus for hard tissue sections

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A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
09:46

A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy

Published on: January 17, 2018

A S Malcolm

    Australian Dental Journal
    |February 1, 1975
    PubMed

    Abstract:

    A Lapping Apparatus is described which enables sections both embedded and unembedded to be ground plano parallel within +/- 1 micron. Sections cemented to steel subplates are retained by a magnetic holder which simplifies loading and unloading. Good surface and edge finish can be obtained and only short lapping times are required.

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    Fixation and Sectioning01:03

    Fixation and Sectioning

    Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
    The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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