Transformation of cells in human bone tumor cultures

Two cell lines from tumors of 16 patients with osteosarcoma and two cell lines from 5 patients with giant cell tumor of bone showed conversion of cell type after 2 to 13 months in culture. Transformed cells of epithelial like morphology appeared in small focal areas and rapidly overgrew nontransformed cells. These cells were characterized by rapid growth, loss of contact inhibition, and growth in soft agar. Attempts to demonstrate virus(es) by electron microscopy, treatment with chemicals, or by inoculation of human cell lines were thus far unsuccessful. Antigens not present in parental cultures were observed in the transformed cells by fixed immunofluorescence test with sera of 13 and 20 osteosarcoma patients and 3 of 8 patients with giant cell tumor of bone. Absorption of positive sera with transformed cells of either osteosarcoma or giant cell tumor removed the reaction but not with absorption with heterophile material or mycoplasma. Presence of group-specific-like antigen (gs-3) in the transformed cells (but not in parent cultures) was shown by immunofluorescence. Fluids of transformed cultures contained heavy RNA similar to that of oncornaviruses. These findings suggest the presence of viral information in some human bone tumors.