Related Experiment Videos
Free thiol groups are essential for infectivity of human cytomegalovirus
Ali Mirazimi1, Mehrdad Mousavi-Jazi1, Vivi-Anne Sundqvist2
1Department of Virology, SMI/Karolinska Institute, 171 82 Solna, Sweden 1.
The Journal of General Virology
|December 2, 1999
Abstract:
The membrane-impermeable thiol blocker 5'5-dithiobis 2- nitrobenzoic acid (DTNB) blocked infectivity of human cytomegalovirus (CMV) although the virus still bound to cells. DTNB-treated CMV regained 65% of its infectivity after incubation with the disulfide bond-reducing agent dithiothreitol. These observations suggest that free thiol groups on CMV are required for infectivity and may participate in disulfide bond formation during virus entry.