Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Non-equilibrium in the Cell

Non-equilibrium in the Cell

An important concept in studying metabolism and energy is that of chemical equilibrium. Most chemical reactions are reversible. They can proceed in both directions, releasing energy into their environment in one direction, and absorbing it from the environment in the other direction. The same is true for the chemical reactions involved in cell metabolism, such as the breaking down and building up of proteins into and from individual amino acids, respectively. Reactants within a closed system...

Cell Motility through Blebbing

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...

Actin Treadmilling

Actin Treadmilling

Actin filaments undergo polymerization and depolymerization from either end. The polymerization and depolymerization rates depend on the cytosolic concentration of free G-actins. The polymerization rate is generally higher at the plus or barbed end, while the depolymerization rate is higher at the minus or pointed end. At a steady state, critical concentration describes the concentration of free G-actin monomers at which the polymerization rate at the plus end is equal to that of the...

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

Maintenance of the ES Cell State

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

Muscle Stimulation Frequency

Muscle Stimulation Frequency

The contraction strength of muscles is regulated by motor neurons, which modulate the frequency of action potentials dispatched to the motor units based on the body's requirements. This process of varying the muscle stimulation frequency allows muscles to contract with a force that is precisely tailored to the needs of the moment, whether lifting a feather or a heavy box.
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

What ecological factors can affect albumen corticosterone levels in the clutches of seabirds? Timing of breeding, disturbance and laying order in rockhopper penguins (Eudyptes chrysocome chrysocome).

General and comparative endocrinology·2009

Same author

Biosimulation of drug metabolism--a yeast based model.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2008

Same author

Next generation adoptive immunotherapy--human T cells as carriers of therapeutic nanoparticles.

Journal of nanoscience and nanotechnology·2008

Same author

Sustained oscillations in glycolysis: an experimental and theoretical study of chaotic and complex periodic behavior and of quenching of simple oscillations.

Biophysical chemistry·2006

Same author

Functionalization and cellular uptake of boron carbide nanoparticles. The first step toward T cell-guided boron neutron capture therapy.

Bioconjugate chemistry·2006

Same author

Preparation and characterization of Boron carbide nanoparticles for use as a novel agent in T cell-guided boron neutron capture therapy.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2005

Search research articles

Related Experiment Video

Updated: Jul 19, 2026

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Published on: July 29, 2007

Sustained oscillations in living cells.

S Danø¹, P G Sørensen, F Hynne

¹Department of Chemistry and CATS, H.C. Orsted Institute, University of Copenhagen, Denmark. sdd@osc.kiku.dk

|December 2, 1999

Summary

Yeast cells can maintain sustained glycolytic oscillations indefinitely using a continuous flow system. This sustained oscillation is mathematically described by the Hopf bifurcation, revealing insights into cellular dynamics.

Area of Science:

Biochemistry
Cellular Dynamics
Systems Biology

Background:

Glycolytic oscillations in yeast have been historically studied using transient glucose pulses.
Previous methods focused on measuring oscillations in NADH, a key metabolic intermediate.
Understanding sustained cellular oscillations is crucial for metabolic research.

Purpose of the Study:

To establish a method for maintaining yeast cells in a sustained, well-defined oscillating state.
To mathematically characterize the transition between stationary and oscillatory metabolic behavior.
To investigate the role of signaling molecules in yeast glycolytic oscillations.

Main Methods:

Utilizing a continuous flow cuvette system to supply starved yeast cells, glucose, and cyanide.

More Related Videos

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Published on: October 4, 2010

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

Related Experiment Videos

Last Updated: Jul 19, 2026

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Published on: July 29, 2007

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Published on: October 4, 2010

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

Observing and analyzing the resulting transient oscillations of NADH.

Applying nonlinear dynamics principles to model the observed metabolic transitions.

Conducting perturbation experiments to assess cellular coupling during oscillations.

Main Results:

Yeast cells can be indefinitely maintained in a sustained oscillating state through continuous nutrient and waste removal.
The transition from stationary to oscillatory behavior is precisely described by the Hopf bifurcation.
Perturbation experiments indicate strong cellular coupling near the transition point, with oscillations occurring within individual cells.
Glucose acts as a signaling molecule, influencing oscillations when the glucose transporter is not saturated.

Conclusions:

A novel method enables sustained glycolytic oscillations in yeast, facilitating detailed kinetic studies.
The Hopf bifurcation provides a robust mathematical framework for understanding metabolic state transitions.
Cellular coupling remains strong near the transition, suggesting intracellular mechanisms drive oscillations.
Glucose and acetaldehyde function as critical signaling molecules in regulating yeast metabolic oscillations.