Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Patterning the zebrafish axial skeleton requires early chordin function.

S Fisher1, M E Halpern

  • 1Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland, USA. fisher@mail1.ciwemb.edu

Nature Genetics
|December 2, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptional regulation using the Q system in transgenic zebrafish.

Methods in cell biology·2016
Same author

Comparative pharmacokinetics and pharmacodynamics of urocortins 1, 2 and 3 in healthy sheep.

British journal of pharmacology·2012
Same author

Approach to high-frequency, cavity-enhanced Faraday rotation in fluids.

Applied optics·2011
Same author

Case report: Maintaining and withdrawing long-term invasive ventilation in a patient with MND/ALS in a home setting.

Palliative medicine·2011
Same author

Satellite vibration measurements with an autodyne CO(2) laser radar.

Applied optics·2010
Same author

Ground-based laser radar measurements of satellite vibrations.

Applied optics·2010

Chordin (chd) is crucial for zebrafish skeletal development, regulating bone morphogenetic protein (BMP) signaling. This study reveals chordin

Area of Science:

  • Developmental biology
  • Molecular biology
  • Zebrafish models

Background:

  • Bone morphogenetic proteins (BMPs) promote ventral cell fates during vertebrate gastrulation.
  • The organizer region secretes BMP antagonists like chordin and noggin to allow dorsal tissue development.
  • While noggin is implicated in preventing osteogenesis at joint formation sites, chordin's role in skeletogenesis was unclear.

Purpose of the Study:

  • To investigate the role of chordin (chd) in skeletal development and patterning in zebrafish.
  • To determine if chordin's function extends beyond gastrulation to later skeletal morphogenesis.
  • To elucidate the molecular mechanisms underlying chordin's influence on bone formation.

Main Methods:

  • Analysis of zebrafish mutants deficient in chordin function.

Related Experiment Videos

  • Gene expression analysis of BMPs and downstream targets in wild-type and mutant zebrafish.
  • Injections of chordin mRNA into early zebrafish embryos to assess rescue effects.
  • Phenotypic analysis of skeletal development, including axial skeletons and fins.

    • Main Results:

    • Zebrafish mutants lacking chordin function showed misexpression of BMP-related genes and ectopic sclerotomal cells.
    • These mutants exhibited defects in fin and caudal skeletal patterning, despite some recovery from initial ventralization.
    • Restoration of chordin levels via mRNA injection rescued gene expression patterns and resulted in normal skeletal development.
    • Genetically mutant fish treated with chordin mRNA developed normal axial skeletons and fins.

    Conclusions:

    • Chordin plays a critical role in the morphogenesis of the adult zebrafish skeleton.
    • Chordin's function during gastrulation is essential for proper skeletal patterning and development.
    • This study highlights a previously unrecognized role for chordin in skeletogenesis, extending its known functions beyond early embryonic patterning.