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[Construction and expression of HLA-DRalpha, DRB1*0401 transgenic mice model]
1Medical Research Laboratory Center, 2nd Affiliated Hospital of Hunan Medical University, Changsha 410011 P.R.China.
Objective:
To construct transgenic mice model on DR alpha and DRB1*0401 of human MHC-II molecules.
Methods:
By microinjection techniques on germ nucleus of zygotes, DRalpha and DRB1*0401 genes were injected into zygotes of C57BL/6 x DBA/1 hybrid mice and transplanted into the oviducts of pseudopregnancy female mice. The integration and expression of exogenous genes in offspring were detected by PCR, Southern blot and Northern blot, RT-PCR analysis.
Results:
There were 5 founders of all injected mice, which had steadily inherited to the fifth generation. It was found that 95 mice were positive by PCR and 68 mice were integrated exogenous gene by Southern blot analysis. DRalpha and DRB1*0401 genes were expressed on spleen and kidney of transgenic mice.
Conclusion:
This experiment on the construction of DRalpha and DRB1*0401 transgenic mice model is a success.