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[The establishment and application of genomic DNA extraction from peripheral blood]
Abstract
Objective:
To establish the method of genomic DNA extraction.
Methods:
Genomic DNA was directly extracted from peripheral blood with the use of potassium iodide.
Results:
The genomic DNA extracted with potassium iodide was of large molecular weight. A(260)/A(280) and A(260)/ A(230) were 1.85 and 2.2 respectively. The extraction efficiency was greater than 90%. The result of PCR for the DNA was good.
Conclusion:
The method is simple, quick, economical and could be used for studying numbers of clinical samples.