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Related Experiment Videos

Anaphylaxis inhibitory factor in IgE-dependent mast cell stimulation.

M I Astorquiza1, S Fernández

  • 1Institute of Immunology, Universidad Austral de Chile, Valdivia, Chile.

Journal of Investigational Allergology & Clinical Immunology
|December 3, 1999
PubMed
Summary

A human serum factor inhibits IgE-mediated mast cell degranulation and tumor necrosis factor-alpha release. This finding suggests a role for the inhibitory factor in both immediate hypersensitivity and later-phase allergic reactions.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Immunoglobulin E (IgE)-mediated reactions are central to immediate hypersensitivity.
  • Mast cells play a critical role in allergic responses through degranulation and mediator release.
  • Tumor necrosis factor-alpha (TNF-alpha) is a key cytokine released by mast cells during anaphylaxis.

Purpose of the Study:

  • To investigate the effect of a previously identified human serum anaphylaxis inhibitory factor on IgE-dependent mast cell TNF-alpha release.
  • To determine the impact of this factor on mast cell degranulation.

Main Methods:

  • Rat peritoneal mast cells and RBL-2H3 cells were sensitized with monoclonal IgE anti-dinitrophenol (anti-DNP).
  • Cells were challenged with dinitrophenol-bovine serum albumin (DNP-BSA) to induce IgE-dependent activation.

Related Experiment Videos

  • TNF-alpha release and mast cell degranulation were measured at various time points.
  • The inhibitory factor's effect was assessed when pre-incubated with IgE or mast cells before antigen challenge.

    • Main Results:

    • Optimal TNF-alpha release occurred at 30 minutes post-challenge in both cell types.
    • The human serum inhibitory factor significantly inhibited TNF-alpha release and reduced mast cell degranulation when applied before antigen challenge.
    • Pre-incubation of mast cells with the factor alone did not induce TNF-alpha release but increased degranulation via a non-IgE-dependent pathway.

    Conclusions:

    • The human serum anaphylaxis inhibitory factor effectively suppresses IgE-dependent TNF-alpha release and degranulation in mast cells.
    • The factor may modulate both immediate hypersensitivity and later-phase allergic inflammatory responses.