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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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DNA technology in the clinical laboratory.
1Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, MI 48073-6769, USA.
Archives of Pathology & Laboratory Medicine
|December 3, 1999
Summary
Advances in molecular biology techniques offer new clinical applications. These methods aid in diagnosing genetic disorders and analyzing cell clonality for improved patient outcomes.
Area of Science:
- Molecular biology
- Clinical pathology
- Genetics
Background:
- Molecular biological techniques are rapidly advancing.
- Clinical applications of these techniques are expanding.
- The Eighth Annual William Beaumont Hospital Symposium highlighted these advances.
Purpose of the Study:
- To review advances in clinically useful molecular biological techniques.
- To identify their applications in clinical practice.
Main Methods:
- Review of 10 submitted manuscripts from a symposium.
- Comparison of findings with existing literature.
- Categorization of manuscripts by topic: nucleic acid stability, analytic approaches, B-/T-cell clonality, and genetic polymorphisms.
Main Results:
- DNA from dried blood spots is stable for analysis.
- Methods exist to stabilize RNA and reduce degradation.
- Genomic amplification techniques improve detection sensitivity.
- Multiplex polymerase chain reaction enables detection of hemochromatosis mutations.
- Southern blotting and PCR detect B-/T-cell clonality in lymphomas.
- Genetic polymorphisms in coagulation factors and platelet glycoproteins are linked to ischemic heart disease.
Conclusions:
- The Human Genome Project will drive the development of more molecular assays.
- Clinical outcome analysis is needed to justify the cost-effectiveness of molecular assays in routine labs.
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