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Related Experiment Videos

Mantle cell lymphoma.

R C Hankin1, S V Hunter

  • 1Department of Anatomic Pathology, William Beaumont Hospital, Royal Oak, MI 48073, USA.

Archives of Pathology & Laboratory Medicine
|December 3, 1999
PubMed
Summary
This summary is machine-generated.

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Diagnosing mantle cell lymphoma (MCL) relies on ancillary immunohistochemical and molecular assays. While cyclin D1 immunohistochemistry is often sufficient, molecular tests for the t(11;14) translocation aid in select cases.

Area of Science:

  • Hematopathology
  • Oncology
  • Molecular Diagnostics

Background:

  • Mantle cell lymphoma (MCL) is an aggressive B-cell neoplasm with a poor prognosis.
  • The 1994 Revised European-American Classification of Lymphoid Neoplasms (REAL) recognizes MCL.
  • The characteristic t(11;14)(q13;q32) translocation is crucial in MCL oncogenesis.

Purpose of the Study:

  • To summarize useful ancillary immunohistochemical and molecular assays for diagnosing mantle cell lymphoma.
  • To review recent advances in molecular characterization and diagnostic test utility for MCL.

Main Methods:

  • Literature survey focusing on recent publications and key advances in MCL molecular characterization.
  • Inclusion of authors' series of 26 small B-cell lymphomas analyzed for cyclin D1 protein and t(11;14) translocation.

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  • Evaluation of immunohistochemistry, polymerase chain reaction (PCR), Southern blot, and fluorescence in situ hybridization (FISH) for diagnostic utility.

    • Main Results:

    • Improved immunohistochemical tests are generally sufficient for confirming MCL diagnosis.
    • Molecular diagnostic tests for t(11;14) vary in sensitivity, specificity, and ease of use.
    • Conventional cytogenetics and PCR for t(11;14) can be helpful but are laborious or have limited sensitivity.

    Conclusions:

    • Mantle cell lymphoma diagnosis benefits from a combination of assays.
    • While immunohistochemistry is often definitive, molecular tests like PCR for t(11;14) have a role in specific scenarios.
    • Further development of methods like FISH is needed for faster, more sensitive MCL diagnosis.