Related Experiment Video

Updated: Aug 16, 2026

Fat-Water Phantoms for Magnetic Resonance Imaging Validation: A Flexible and Scalable Protocol
07:59

Fat-Water Phantoms for Magnetic Resonance Imaging Validation: A Flexible and Scalable Protocol

Published on: September 7, 2018

Protein-water interaction studied by solvent 1H, 2H, and 17O magnetic relaxation

S H Koenig, K Hallenga, M Shporer

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1975
    PubMed

    More Related Videos

    Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate11:57

    Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate

    Published on: September 13, 2019

    Using Solution NMR to Characterize Biomolecular Condensates Under Biphasic Conditions09:01

    Using Solution NMR to Characterize Biomolecular Condensates Under Biphasic Conditions

    Published on: April 17, 2026

    Related Concept Videos

    ¹H NMR: Interpreting Distorted and Overlapping Signals01:02

    ¹H NMR: Interpreting Distorted and Overlapping Signals

    Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
    As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
    ¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

    ¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

    At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
    ¹H NMR of Labile Protons: Temporal Resolution01:10

    ¹H NMR of Labile Protons: Temporal Resolution

    Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
    The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...
    ¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

    ¹H NMR of Labile Protons: Deuterium (²H) Substitution

    This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
    ¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

    ¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

    The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
    Atomic Nuclei: Types of Nuclear Relaxation01:28

    Atomic Nuclei: Types of Nuclear Relaxation

    Nuclear relaxation restores the equilibrium population imbalance and can occur via spin–lattice or spin–spin mechanisms, which are first-order exponential decay processes.
    In spin–lattice or longitudinal relaxation, the excited spins exchange energy with the surrounding lattice as they return to the lower energy level. Among several mechanisms that contribute to spin–lattice relaxation, magnetic dipolar interactions are significant. Here, the excited nucleus transfers energy to a nearby...