Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Establishment of tight junctions between epithelial cells.

A J Hudspeth

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1975
    PubMed
    Summary

    Epithelial cells rapidly restore barrier function after injury. New tight junctions form within 30 minutes, ensuring tissue integrity and preventing solute diffusion.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Optogenetic interrogation of the zebrafish lateral line reveals brain-wide neural circuits involved in pattern separation.

    iScience·2025
    Same author

    Axonal defasciculation is restricted to specific branching points during regeneration of the lateral line nerve in zebrafish.

    Development (Cambridge, England)·2025
    Same author

    <i>ybx1</i> acts upstream of <i>atoh1a</i> to promote the rapid regeneration of hair cells in zebrafish lateral-line neuromasts.

    PeerJ·2025
    Same author

    Axonal defasciculation is restricted to specific branching points during regeneration of the lateral line nerve in zebrafish.

    bioRxiv : the preprint server for biology·2025
    Same author

    Amplification through local critical behavior in the mammalian cochlea.

    Proceedings of the National Academy of Sciences of the United States of America·2025
    Same author

    The Price and Quality of Methylphenidate Products.

    Journal of the American Academy of Child and Adolescent Psychiatry·2025

    Area of Science:

    • Cell biology
    • Physiology
    • Epithelial biology

    Background:

    • Epithelia act as crucial barriers between body compartments.
    • Maintaining epithelial integrity is vital despite continuous cell loss.
    • Rapid repair mechanisms are essential for tissue homeostasis.

    Purpose of the Study:

    • To investigate the speed and mechanisms of epithelial repair after cell removal.
    • To determine the timeframe for restoring barrier function and tight junction formation.
    • To demonstrate rapid restoration of epithelial integrity.

    Main Methods:

    • Experimental removal of single cells from Necturus gallbladder epithelium.
    • Electrophysiological measurements to assess electrical resistance.
    • Electron microscopy to observe tight junction formation.

    Main Results:

    • Contiguous epithelial cells migrated to fill experimentally created defects within 30 minutes.
    • Electrical resistance across the wounded epithelium returned to normal levels in 30 minutes.
    • Morphologically recognizable and physiologically functional tight junctions were formed concurrently with wound closure.

    Conclusions:

    • Epithelial tissues possess a rapid repair mechanism to restore integrity after cell loss.
    • Physiologically functional tight junctions can be established within 30 minutes, ensuring barrier function.
    • This rapid restoration highlights an efficient biological process for maintaining epithelial barriers.

    Related Experiment Videos