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Membrane chromatography: preparation and applications to protein separation
1Department of Chemical Engineering, State University of New York at Buffalo, Amherst, New York 14260, USA.
Biotechnology Progress
|December 10, 1999
Summary
Membrane chromatography offers superior protein and enzyme purification compared to traditional methods. This review details adsorptive membrane preparation, focusing on novel chitin and chitosan membranes for enhanced separations.
Area of Science:
- Biochemical Engineering
- Separation Science
- Materials Science
Background:
- Membrane chromatography utilizes convective flow for efficient solute capture, outperforming column chromatography in productivity.
- Industrial applications are significant for protein and enzyme recovery, isolation, and purification.
Purpose of the Study:
- To provide a comprehensive review of adsorptive membrane preparation techniques.
- To highlight novel macroporous chitin and chitosan membranes for protein separations.
Main Methods:
- Review of established adsorptive membrane preparation methods: surface modification, in situ copolymerization, direct formation, and functionalized particulate-entrapped membranes.
- Development and characterization of novel macroporous chitin and chitosan membranes.
Main Results:
- Adsorptive membranes demonstrate higher capture efficiency and productivity due to convective flow.
- Chitin and chitosan membranes show promise for advanced protein separation applications.
Conclusions:
- Membrane chromatography is a highly efficient technique for bioseparations.
- Novel chitin and chitosan membranes represent a significant advancement in adsorptive separation technology.