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Related Experiment Videos

Rice bran lipase: extraction, activity, and stability

Prabhu1, Tambe, Gandhi

  • 1Department of Chemical Technology, University of Mumbai, Matunga, Mumbai 400019, India.

Biotechnology Progress
|December 10, 1999
PubMed
Summary

Researchers developed a simple procedure to extract lipase from rice bran, optimizing conditions for maximum yield. This method enhances enzyme activity and stability for potential industrial applications.

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Area of Science:

  • Biochemistry
  • Enzyme Technology
  • Agricultural Science

Background:

  • Rice bran is a rich source of bioactive compounds, including enzymes.
  • Lipases are crucial enzymes with diverse industrial applications.
  • Efficient extraction and stabilization of lipases are essential for their utilization.

Purpose of the Study:

  • To develop a simple and efficient procedure for extracting lipolytic activity from rice bran.
  • To optimize extraction conditions for maximum lipase yield and activity.
  • To investigate the stability of rice bran lipase and the effect of glycerol as a stabilizer.

Main Methods:

  • Rice bran was defatted to remove lipids.
  • Aqueous extraction was performed using potassium phosphate buffer (50 mM, pH 7) with CaCl(2).

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  • Micro- and ultrafiltration were used for further purification.

    • Main Results:

    • Defatting rice bran significantly increased enzyme activity.
    • Five cycles of aqueous extraction yielded maximum lipase.
    • Glycerol enhanced enzyme stability under storage and operative conditions.
    • Purification via micro- and ultrafiltration resulted in higher specific activity and stability.

    Conclusions:

    • A straightforward extraction protocol for rice bran lipase has been established.
    • Optimized conditions and glycerol stabilization improve enzyme yield and performance.
    • Purified rice bran lipase exhibits enhanced activity and stability, making it suitable for further applications.