Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence requirements for plasmid nuclear import.

D A Dean1, B S Dean, S Muller

  • 1College of Medicine, University of South Alabama, Mobile, Alabama 36688, USA. dean@sungcg.usouthal.edu

Experimental Cell Research
|December 10, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Etiology of lipid-laden macrophages in the lung.

International immunopharmacology·2023
Same author

The CHALLENGE trial: the effects of a virtual reality-assisted exposure therapy for persistent auditory hallucinations versus supportive counselling in people with psychosis: study protocol for a randomised clinical trial.

Trials·2022
Same author

Decreasing surface albedo signifies a growing importance of clouds for Greenland Ice Sheet meltwater production.

Nature communications·2022
Same author

Seventy years of data from the world's longest grazed and irrigated pasture trials.

Scientific data·2021
Same author

Preferential transcription of the mutated allele in NPM1 mutated acute myeloid leukaemia.

Scientific reports·2020
Same author

Mapping of host-parasite-microbiome interactions reveals metabolic determinants of tropism and tolerance in Chagas disease.

Science advances·2020
Same journal

The AP2α/PDHA1 signaling pathway promotes lung cancer progression by mediating aerobic glycolysis and inhibiting cuproptosis.

Experimental cell research·2026
Same journal

Distributed transcription activity in DLX proteins defies conventional mapping of a transcription activation domain.

Experimental cell research·2026
Same journal

Extracellular release of cytotoxic aggregates from HSV-1-replicating SH-SY5Y cells: involvement of alpha-synuclein and poly-ubiquitin conjugates.

Experimental cell research·2026
Same journal

MiR-1281 downregulates LMX1B to inhibit gastric cancer development.

Experimental cell research·2026
Same journal

Corrigendum to "Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells" [Exp. Cell Res., 326, 1, 1 August 2014, Pages 78-89].

Experimental cell research·2026
Same journal

Neutrophilic differentiation of promyelocytic HL-60 cells is associated with increased expression of terminal galactose residues on the cell surface.

Experimental cell research·2026
See all related articles

The SV40 enhancer sequence significantly improves plasmid DNA nuclear import for nonviral gene delivery. This sequence facilitates maximal transport, overcoming the nuclear envelope barrier, especially in non-dividing cells.

Area of Science:

  • Molecular Biology
  • Gene Delivery
  • Cell Biology

Background:

  • The nuclear envelope poses a significant barrier to nonviral gene delivery.
  • Efficient nuclear uptake of plasmid DNA is crucial for gene expression.

Purpose of the Study:

  • To identify specific DNA sequences that enhance nuclear import of plasmids.
  • To evaluate the role of the SV40 enhancer in plasmid nuclear localization.

Main Methods:

  • Investigated nuclear import of plasmid DNA fragments in cells.
  • Assessed the role of SV40 enhancer, origin of replication, and promoter.
  • Compared SV40 enhancer with CMV and Rous sarcoma virus LTR sequences.
  • Measured green fluorescent protein (GFP) expression in microinjected cells.

Related Experiment Videos

Main Results:

  • The 72-bp repeats of the SV40 enhancer significantly facilitated maximal nuclear transport of plasmids.
  • SV40 origin of replication and promoter functions were not required for nuclear localization.
  • CMV and Rous sarcoma virus LTR sequences failed to direct plasmid nuclear localization.
  • SV40 enhancer-containing plasmids showed significant GFP expression in cytoplasmically injected cells, particularly before cell division.

Conclusions:

  • The SV40 enhancer sequence is a key determinant for efficient nuclear import of plasmids.
  • Incorporating the SV40 enhancer into nonviral vectors can enhance gene delivery, especially in non-dividing cells.