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A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
12:55

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Published on: December 9, 2021

Amoxycillin/clavulanic acid combinations increase transmigration of leucocytes through endothelial cell monolayers:

R Hofbauer1, D Moser, B Gmeiner

  • 1Department of Medical and Chemical Laboratory Diagnostics, University of Vienna, Austria. roland.hofbauer@akh-wien.ac.at

The Journal of Antimicrobial Chemotherapy
|December 10, 1999
PubMed

Insights

The antibiotic amoxicillin/clavulanic acid (co-amoxiclav) significantly increases polymorphonuclear neutrophil leucocyte (PMNL) migration through endothelial cell monolayers (ECMs). This effect is most pronounced when both PMNLs and ECMs are treated, highlighting co-amoxiclav

Area of Science:

  • Immunology
  • Pharmacology
  • Cell Biology

Background:

  • Postoperative inflammation remains a significant clinical challenge.
  • Leukocyte migration into tissues is a key inflammatory process.
  • Agents like anesthetics can modulate leukocyte recruitment.

Purpose of the Study:

  • To investigate the effect of amoxicillin/clavulanic acid (co-amoxiclav) on leukocyte migration.
  • To determine how co-amoxiclav influences polymorphonuclear neutrophil leucocyte (PMNL) transmigration through endothelial cell monolayers (ECMs).

Main Methods:

  • Human umbilical endothelial cells were cultured to form monolayers (ECMs) on microporous membranes.
  • A migration assay was performed using PMNLs.
  • PMNLs and/or ECMs were pretreated with varying concentrations of co-amoxiclav.

Main Results:

  • Co-amoxiclav significantly increased PMNL migration through ECMs (P<0.05) when both PMNLs and ECMs were treated (291+/-18.7%).
  • Treatment of ECMs alone had a greater effect on migration than treatment of PMNLs alone.
  • The most significant increase in transmigration occurred when both PMNLs and ECMs received co-amoxiclav treatment.

Conclusions:

  • Co-amoxiclav is a potent agent that enhances leukocyte transmigration across endothelial cell monolayers.
  • Endothelial cell monolayers play a critical role in co-amoxiclav's effect on leukocyte migration.
  • Co-amoxiclav demonstrates an impact on endothelial cell function.

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