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Oxidized LDL, glomerular mesangial cells and collagen
1Department of Pathology, Seoul National University College of Medicine, South Korea. hyunsoon@plaza.snu.ac.kr
Diabetes Research and Clinical Practice
|December 10, 1999
Summary
Oxidized low-density lipoprotein (Ox-LDL) contributes to glomerulosclerosis by promoting mesangial matrix expansion. Antioxidants and Ox-LDL antibodies reduce this effect, suggesting therapeutic potential for kidney disease.
Area of Science:
- Nephrology
- Biochemistry
- Pathology
Background:
- Focal segmental glomerulosclerosis (FSGS) involves lipid deposits, foam cells, and mesangial matrix accumulation.
- Oxidative stress and oxidized low-density lipoprotein (Ox-LDL) are implicated in uremic conditions and FSGS lesions.
Purpose of the Study:
- To investigate the role of oxidized low-density lipoprotein (Ox-LDL) in FSGS pathogenesis.
- To determine the effect of antioxidants and Ox-LDL antibodies on collagen production in human mesangial cells (HMC).
Main Methods:
- In vivo studies using rats with experimental FSGS treated with dietary antioxidants.
- In vitro studies exposing human mesangial cells (HMC) to low-density lipoprotein (LDL).
- Assessing the impact of vitamin E and anti-Ox-LDL antibody on collagen mRNA expression.
Main Results:
- Dietary antioxidants reduced Ox-LDL staining and renal injury in experimental FSGS.
- LDL modified in vitro stimulated collagen mRNA expression in HMC.
- Vitamin E and anti-Ox-LDL antibody significantly reduced LDL-induced collagen mRNA.
Conclusions:
- Deposited and oxidized LDL in glomeruli may drive glomerulosclerosis.
- Ox-LDL promotes mesangial matrix generation, contributing to FSGS development.
- Antioxidant strategies may offer a protective approach against FSGS progression.
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