Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nutrient absorption.

D F Nutting1, N S Kumar, R J St Hilaire

  • 1University of Tennessee-Memphis, Department of Medicine 38163, USA.

Current Opinion in Clinical Nutrition and Metabolic Care
|December 10, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The landscape of conventional and artificial intelligence-based clinical prediction models in non-small-cell lung cancer: from development to real-world validation.

ESMO open·2025
Same author

Chitosan as an environment friendly biomaterial - a review on recent modifications and applications.

International journal of biological macromolecules·2019
Same author

Effect of sintering temperature on structural and optical properties of indium(III) oxide nanoparticles prepared with Triton X-100 by hydrothermal method.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2014
Same author

Basaloid squamous cell carcinoma of retromolar trigone: A case report with review of literature.

Journal of oral and maxillofacial pathology : JOMFP·2012
Same author

Breakthrough of toluene vapours in granular activated carbon filled packed bed reactor.

Journal of hazardous materials·2009
Same author

Concepts and strategies for scaling up focused prevention for sex workers in India.

Sexually transmitted infections·2008

Recent advances reveal that endoplasmic reticulum-to-Golgi transport limits lipid absorption. Apolipoprotein B aids particle movement, while peptide transporters absorb modified peptides and leptin receptors in the intestine modulate body mass.

Area of Science:

  • Nutrient absorption
  • Lipid metabolism
  • Molecular biology

Background:

  • Endoplasmic reticulum-to-Golgi transport is a critical step in lipid absorption.
  • Apolipoprotein B's role in chylomicron formation and transport is under investigation.
  • Drug bioavailability can be enhanced through esterification, but absorption mechanisms require clarification.
  • Leptin's systemic effects may involve direct modulation of intestinal nutrient absorption.
  • Gene regulation of apolipoprotein B differs between the liver and intestine.

Purpose of the Study:

  • To investigate the rate-limiting step in lipid absorption.
  • To elucidate the function of Apolipoprotein B in lipid particle transport.
  • To explore the absorption pathways for modified peptides and drugs.
  • To identify novel roles for leptin in the gastrointestinal tract.

Related Experiment Videos

  • To compare the gene regulation of apolipoprotein B in different tissues.

    • Main Methods:

    • Kinetics of triacylglycerol appearance in cellular compartments and lymph.
    • Analysis of chylomicron-sized lipid particle formation and transport.
    • In vitro studies using human ileal mucosa.
    • Investigation of peptide and drug absorption using peptide transporters.
    • Assessment of leptin receptor presence and function in intestinal mucosa.

    Main Results:

    • Endoplasmic reticulum-to-Golgi transport appears to be the rate-limiting step for lipid absorption.
    • Apolipoprotein B is essential for the transport of lipid particles from the endoplasmic reticulum to the Golgi, not their initial formation.
    • Fatty acylation of peptides and esterification of acyclovir with valine do not prevent absorption via peptide transporters.
    • Human intestinal mucosa possesses leptin receptors, and leptin inhibits galactose absorption.
    • The enhancer element for the apolipoprotein B gene is located significantly farther from its structural gene in the intestine compared to the liver.

    Conclusions:

    • Lipid absorption is regulated by the rate of transport between the endoplasmic reticulum and Golgi apparatus.
    • Apolipoprotein B plays a crucial role in the post-formation trafficking of lipid particles.
    • Peptide transporters are capable of absorbing modified peptides and esterified drugs.
    • Leptin may directly influence nutrient absorption and body mass regulation via intestinal receptors.
    • Tissue-specific differences in apolipoprotein B gene regulation exist, particularly concerning enhancer element location.