Related Experiment Video
Updated: Jun 19, 2026

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
Published on: February 15, 2012
A novel member of the subtilisin-like protease family from Bacillus subtilis
Angela Valbuzzi1, Eugenio Ferrari2, Alessandra M Albertini1
1Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, Via Abbiategrasso, 207, 27100 Pavia, Italy1.
Abstract:
aprX is a 1326 bp gene of Bacillus subtilis strain 168 that encodes a serine protease, probably intracellular, characterized by significant similarity with subtilisins, thermitases and pyrolysins. Transcription analysis, performed by RT-PCR and primer extension, allowed the localization of the active promoter and showed that aprX is expressed in stationary phase. The pattern of expression of aprX and its dependence on various transition state regulatory genes (degU, degQ, hpr, abrB, sinR), monitored by lacZ transcriptional fusions, are distinctive from those of subtilisin and other degradative enzymes. aprX is not essential for either growth or sporulation.
Related Concept Videos
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The Proteasome Structure
The proteasome is an...
Gram-negative Bacterial Protein Secretion Systems
Bacterial Phylum Verrucomicrobiota
Production of Biopesticides
Bacterial Toxins

