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Updated: Oct 18, 2026

Ganglioside Extraction, Purification and Profiling
Published on: March 12, 2021
Gangliosides are transported from the plasma membrane to intralysosomal membranes as revealed by immuno-electron
W Möbius1, V Herzog, K Sandhoff
1Kekulé-Institute für Organische Chemie und Biochemie, Bonn, Germany.
Abstract:
A biotin-labeled derivative of the ganglioside GM1 (biotin-GM1) was used to study its transport along the endocytic pathway of cultured fibroblasts by immuno-electron microscopy. Using electron dense endocytic tracers we could demonstrate that late endosomes and lysosomes of these cells are long living organelles with a high content of internal membranes. Our studies show that during endocytosis the biotin-GM1 was transported to these intraendosomal and intralysosomal membranes. These observations support the hypothesis that glycosphingolipids (GSL) are preferentially degraded in intralysosomal vesicles.
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