Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Notch2 expression negatively correlates with glial differentiation in the postnatal mouse brain.

M Tanaka1, Y Kadokawa, Y Hamada

  • 1Division of Cell Biology, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan.

Journal of Neurobiology
|December 10, 1999
PubMed
Summary

Notch2 is expressed by glial cells in the postnatal mouse brain, not just neurons. This finding suggests Notch2 acts as a negative regulator of glial differentiation during mammalian brain development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A TBP complex essential for transcription from TATA-less but not TATA-containing RNA polymerase III promoters is part of the TFIIIB fraction.

Cell·1992
Same author

A novel orally active inhibitor of IL-1 generation: synthesis and structure-activity relationships of 3-(4-hydroxy-1-naphthalenyl)-2-propenoic acid derivatives.

Journal of medicinal chemistry·1992
Same author

[An autopsy case of ACTH producing prostate neoplasms with Cushing's syndrome].

Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·1992
Same author

Intraoperative endoscopic electrohydraulic lithotripsy of pancreatic stones.

International journal of pancreatology : official journal of the International Association of Pancreatology·1992
Same author

[A survey of body temperatures in the elderly and the thermal environment in nursing homes].

[Nihon koshu eisei zasshi] Japanese journal of public health·1992
Same author

Isolation and identification of a cytotoxic principle from Chrysosplenium grayanum Maxim. (Saxifragaceae) and its antitumor activities.

Chemical & pharmaceutical bulletin·1992

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Notch family molecules are recognized as negative regulators of neuronal differentiation during early brain development.
  • Notch2 expression persists in specific postnatal rodent brain regions after embryonic expression.

Purpose of the Study:

  • To investigate Notch2 expression patterns in the postnatal mouse brain.
  • To determine the cell types expressing Notch2 in postnatal brain regions with ongoing neurogenesis.
  • To explore the functional role of Notch2 in glial cell differentiation.

Main Methods:

  • Utilized lacZ knockin mice to track Notch2 expression in the postnatal brain.
  • Performed double and triple staining on brain sections and dissociated cell cultures.

Related Experiment Videos

  • Analyzed glial gene expression (vimentin, GFAP) and bromodeoxyuridine incorporation.

    • Main Results:

    • Notch2 expression was detected in the developing cerebellum and hippocampus, regions of persistent postnatal neurogenesis.
    • Notch2 was found in Bergmann glia, radial glia, and astrocytes.
    • Notch2 expression in glial cells correlated with immature glial gene expression and increased proliferation (bromodeoxyuridine incorporation).

    Conclusions:

    • Notch2 is expressed by glial cells in the postnatal mammalian brain.
    • Notch2 expression negatively correlates with glial differentiation.
    • Proposes a novel role for Notch2 as a negative regulator of glial differentiation in mammalian brain development.