Transcription of some PHO genes in Saccharomyces cerevisiae is regulated by spt7p
K Nishimura1, K Yasumura, K Igarashi
1Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Abstract:
Spt7p is a new global transcription factor in Saccharomyces cerevisiae(Gansheroff et al., 1995). We report here that the activities of high affinity phosphate transport and acid phosphatase in particular were decreased in a spt7 null mutant. Northern blot experiments revealed that transcription of the PHO84 and PHO5 genes was impaired in this mutant; expression of the PHO regulatory genes, PHO4 and PHO2, was normal. Spt7p is thus linked with expression of several structural genes of the PHO regulon in yeast.
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