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The temporal requirement for endothelin receptor-B signalling during neural crest development
M K Shin1, J M Levorse, R S Ingram
1Howard Hughes Medical Institute and Department of Molecular Biology, Princeton University, New Jersey 08544, USA.
Endothelin receptor B (EDNRB) is crucial for melanocyte and neuron development. This study shows EDNRB signaling is essential during a specific window of mouse embryonic development for cell migration.
Area of Science:
- Developmental biology
- Genetics
- Neuroscience
Background:
- Endothelin receptor B (EDNRB) is a G-protein-coupled receptor vital for melanocyte and enteric neuron development.
- Mutations in the Ednrb gene in mice lead to white coat color and lethal megacolon.
Purpose of the Study:
- To pinpoint the critical timing of EDNRB signaling during embryogenesis.
- To investigate the role of EDNRB in neural crest development.
Main Methods:
- Utilized a tetracycline-inducible system to control endogenous Ednrb gene expression in mice.
- Generated mouse strains expressing Ednrb at specific embryonic stages.
Main Results:
- EDNRB signaling is required during a narrow window of neural crest development (embryonic days 10-12.5).
- EDNRB is essential for the migration of both melanoblasts and enteric neuroblasts.
Conclusions:
- EDNRB plays a critical, time-sensitive role in embryonic development.
- Proper EDNRB function is necessary for the migration of key cell types during neural crest formation.
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