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Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
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The RCAF complex mediates chromatin assembly during DNA replication and repair

J K Tyler1, C R Adams, S R Chen

  • 1Department of Biology and Center for Molecular Genetics, University of California at San Diego, La Jolla 92093-0347, USA.

Nature
|December 11, 1999
PubMed
Summary

Researchers identified a new protein complex, replication-coupling assembly factor (RCAF), crucial for building nucleosomes on replicated DNA. RCAF, containing ASF1 and histones H3/H4, is vital for cell cycle progression and DNA repair.

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