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Related Experiment Videos

Myelin structure transformed by dimethylsulfoxide.

D A Kirschner, D L Caspar

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1975
    PubMed
    Summary

    Dimethyl sulfoxide (DMSO) induces a new, highly ordered nerve structure by reducing water content. This transformation is reversible, with native myelin structure reappearing when DMSO is removed.

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    Area of Science:

    • Neuroscience
    • Biophysics
    • Materials Science

    Background:

    • Myelin, a lipid-rich sheath, is crucial for nerve impulse conduction.
    • Understanding myelin's structural dynamics is key to neurological research.

    Purpose of the Study:

    • To investigate the structural effects of dimethyl sulfoxide (DMSO) on nerve myelin.
    • To characterize the novel structure induced by DMSO and its reversibility.

    Main Methods:

    • X-ray diffraction was used to analyze nerve samples.
    • Nerves were exposed to varying concentrations of DMSO in Ringer's solution.

    Main Results:

    • DMSO (≥10%) induced a new, highly ordered structure with a shorter repeat period.
    • Higher DMSO concentrations led to greater myelin transformation.
    • The native myelin structure rapidly reappeared upon DMSO removal.
    • DMSO's effect is attributed to water loss from intermembrane spaces, preserving the bilayer structure.

    Conclusions:

    • Dimethyl sulfoxide induces a reversible, water-dependent structural change in nerve myelin.
    • This finding offers insights into myelin's structural plasticity and response to dehydration.

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