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Related Experiment Videos

MAGEST: MAboya gene expression patterns and sequence tags.

T Kawashima1, S Kawashima, M Kanehisa

  • 1Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.

Nucleic Acids Research
|December 11, 1999
PubMed
Summary

MAGEST is a new database detailing maternal messenger RNAs (mRNAs) in the ascidian Halocynthia roretzi. It provides valuable gene expression data for early embryonic development research.

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Area of Science:

  • Developmental Biology
  • Genomics
  • Marine Biology

Background:

  • Ascidian maternal mRNAs play crucial roles in early embryonic development.
  • Understanding mRNA populations is key to deciphering developmental processes.
  • Halocynthia roretzi serves as a model organism for marine invertebrate development.

Purpose of the Study:

  • To establish MAGEST, a comprehensive database of maternal cDNAs in Halocynthia roretzi.
  • To catalog mRNA tag sequences and their expression patterns.
  • To facilitate research on gene expression during early ascidian embryogenesis.

Main Methods:

  • Collection of 3' and 5' tag sequences from Halocynthia roretzi mRNAs.
  • Generation of expression data using whole-mount in situ hybridization in early embryos.

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  • Curation and input of over 2000 cDNA tag sequences into public databases.

    • Main Results:

    • MAGEST database successfully compiled over 2000 maternal cDNA tag sequences for H. roretzi.
    • Expression data from in situ hybridization experiments are available for these sequences.
    • The database provides access to mRNA tag sequences and associated expression information.

    Conclusions:

    • MAGEST offers a valuable resource for studying maternal gene expression in Halocynthia roretzi.
    • The database supports research into the molecular mechanisms of ascidian early development.
    • Accessible online data promotes further investigation into ascidian genomics and developmental biology.