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Colorimetric method for susceptibility testing of voriconazole and other triazoles against Candida species
1Department of Internal Medicine, Ann Arbor Veterans Affairs Health System, University of Michigan Medical 48105, USA. ckauff@umich.edu
Abstract:
A microdilution assay using Alamar Blue, a colorimetric indicator, was compared with the NCCLS macrodilution broth assay for voriconazole, fluconazole, and itraconazole against Candida albicans, Candida glabrata, and Candida krusei. Concordance (+/- 2 dilutions) between the two methods was highest for voriconazole (98.3%), and for fluconazole and itraconazole it was 94.3 and 95.4%, respectively. Twenty-six of 32 (81.2%) discordant readings (> or = 3 dilutions different) were noted in C. glabrata isolates, and all but two isolates showing discordance had higher minimum inhibitory concentration readings with the colorimetric method.