[Isolation of bacteria which could perform nitrification and denitrification simultaneously by gene probe]
Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi = Chinese Journal of Microbiology and Immunology
|December 11, 1999
Summary
Two bacterial strains, Alcaligenes faecalis subsp. faecalis, were isolated for simultaneous nitrification and denitrification of nitrogen waste. Strain 2 demonstrated superior denitrification capabilities under various conditions.
Area of Science:
- Environmental Microbiology
- Biotechnology
- Wastewater Treatment
Context:
- Nitrogen-containing waste poses environmental challenges.
- Simultaneous nitrification and denitrification (SND) is an efficient wastewater treatment process.
- Isolation of effective microbial strains is crucial for optimizing SND.
Purpose:
- To isolate and identify bacteria capable of simultaneous nitrification and denitrification (SND) from nitrogen-containing wastes in Taiwan.
- To investigate the effects of environmental factors (pH, oxygen) and chemical agents on the SND activity of the isolates.
- To compare the SND performance of the isolated strains.
Summary:
- Two bacterial isolates, identified as Alcaligenes faecalis subsp. faecalis strain 1 and strain 2, were obtained using DNA probes from Thiosphaera pantotropha.
- Both isolates exhibited nitrification and denitrification capabilities, even under aerobic conditions.
- Alcaligenes faecalis subsp. faecalis strain 2 showed enhanced denitrification, with activity inhibited by potassium cyanide and nitrification by hydrazine and hydroxyamine.
Impact:
- Provides novel bacterial strains for potential application in advanced wastewater treatment.
- Offers insights into the physiological characteristics and control mechanisms of SND bacteria.
- Contributes to the development of more sustainable and efficient nitrogen removal technologies.
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