[Cloning and sequences comparison of promoters from Aspergillus niger]

An Aspergillus niger (A. niger) genomic library was constructed in a promoter-trap vector, which contains a Hygromycin B (Hy) phosphotransferase-encoding gene (hph) and screened for DNA fragments with promoter activity by applying the sib selection procedure. A functional promoter PX27 was identified. Both DNA strands of this fragment were sequenced and showed no significant homology to the sequence already in the database. Comparison of the sequences of all known promoters from A. niger revealed a consensus CTTCTC, as a novel motif of the A. niger promoters.