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Updated: Aug 13, 2026

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
First-generation physical map of the Culicoides variipennis (Diptera: Ceratopogonidae) genome
R A Nunamaker1, S E Brown, L E McHolland
1Arthropod-Borne Animal Diseases Research Laboratory, USDA-ARS, Laramie, WY 82071, USA.
Abstract:
Recombinant cosmids labeled with biotin-11-dUTP or digoxigenin by nick translation were used as in situ hybridization probes to metaphase chromosomes of Culicoides variipennis (Coquillett). Paired fluorescent signals were detected on each arm of sister chromatids and were ordered along the 3 chromosomes. Thirty-three unique probes were mapped to the 3 chromosomes of C. variipennis (2n = 6): 7 to chromosome 1, 20 to chromosome 2, and 6 to chromosome 3. This work represents the first stage in generating a physical map of the genome of C. variipennis.
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