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Analysis of porcine cytomegalovirus DNA polymerase by consensus primer PCR
V Rupasinghe1, T Tajima, K Maeda
1Department of Veterinary Microbiology, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
Abstract:
We used a consensus primer PCR method to amplify a region of herpesviral DNA-directed DNA polymerase gene using degenerate primers for initial characterization of the porcine cytomegalovirus (PCMV) genome. The sequence of the PCR product from PCMV DNA template and its alignment with other herpesvirus DNA polymerase counterparts showed that both conserved amino acid residues and conservative amino acid substitutions are in parallel. Phylogenetic analysis revealed that PCMV should be included in the clade comprising human herpesvirus 6 and 7, rather than human and mouse cytomegaloviruses, in Betaherpesvirus subfamily.