Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

BUBR1 phosphorylation is regulated during mitotic checkpoint activation.

W Li1, Z Lan, H Wu

  • 1Department of Internal Medicine, University of Cincinnati College of Medicine, Ohio 45267, USA.

Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research
|December 11, 1999
PubMed
Summary

The BUBR1 protein, crucial for the mitotic checkpoint, is regulated by phosphorylation during spindle disruption in human cells. Its expression and phosphorylation status change throughout the cell cycle, suggesting a key role in cell division control.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stimulation of Hyaluronan synthetase by platelet-derived growth factor bb in human prostate smooth muscle cells.

Pharmacology·2001
Same author

Analogs of the antimicrobial peptide trichogin having opposite membrane properties.

European journal of biochemistry·2001
Same author

Estimate of the frequency of true incomplete exchanges in human lymphocytes exposed to 1 GeV/u Fe ions in vitro.

International journal of radiation biology·2001
Same author

Genetic deletion of the Pten tumor suppressor gene promotes cell motility by activation of Rac1 and Cdc42 GTPases.

Current biology : CB·2001
Same author

Mammotome core biopsy for mammary microcalcification: analysis of 160 biopsies from 142 women with surgical and radiologic followup.

Cancer·2001
Same author

Clinical and angiographic outcome in the laser angioplasty for restenotic stents (LARS) multicenter registry.

Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions·2001

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Eukaryotic cells possess a mechanism to delay mitosis until chromosomes align correctly on the spindle.
  • Understanding this mitotic checkpoint is vital for comprehending cell division regulation.

Purpose of the Study:

  • To investigate the molecular basis of the mitotic checkpoint in human cells.
  • To characterize the BUBR1 (also known as MAD3-like protein) gene and its protein product.

Main Methods:

  • Cloning of full-length cDNA for BUBR1.
  • Dot blot and Western blot analyses to assess BUBR1 mRNA and protein expression.
  • Cell cycle analysis and phosphatase treatment to study BUBR1 phosphorylation.

Main Results:

Related Experiment Videos

  • BUBR1 mRNA is found in tissues with high mitotic activity.
  • BUBR1 protein (120 kDa) is expressed across cell cycle stages, with increased levels in late S and G2 phases.
  • BUBR1 undergoes phosphorylation during spindle disruption, indicated by altered mobility on SDS gels.

Conclusions:

  • BUBR1 phosphorylation is regulated during spindle disruption, suggesting its involvement in the mitotic checkpoint.
  • BUBR1's homology to BUB1 protein kinase implies a role in phosphorylating key mitotic checkpoint components.