Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An optical sectioning programmable array microscope implemented with a digital micromirror device.

Q S Hanley1, P J Verveer, M J Gemkow

  • 1Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, D-37070 Göttingen, Germany.

Journal of Microscopy
|December 14, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

When R  >  0.8R <sub>0</sub>: fluorescence anisotropy, non-additive intensity, and cluster size.

Methods and applications in fluorescence·2017
Same author

Elevated α-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells.

Cell death & disease·2015
Same author

Alpha-Synuclein affects neurite morphology, autophagy, vesicle transport and axonal degeneration in CNS neurons.

Cell death & disease·2015
Same author

Visualization of lipid-receptor interactions on single cells by time-resolved imaging fluorescence microscopy.

Journal of fluorescence·2013
Same author

Effects of Oxidation Agents and Metal Ions on Binding of p53 to Supercoiled DNA.

Journal of biomolecular structure & dynamics·2012
Same author

Minimizing light exposure with the programmable array microscope.

Journal of microscopy·2010
Same journal

BioImageIT: A novel python-based architecture for reproducible bio-image workflows.

Journal of microscopy·2026
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
See all related articles

Programmable array microscopes (PAMs) use spatial light modulators for optical sectioning, offering flexible illumination patterns. PAMs show promise for biological imaging, with higher signal and dynamic range than confocal microscopy.

Area of Science:

  • Microscopy
  • Optical Imaging
  • Biophysics

Background:

  • Programmable array microscopes (PAMs) utilize spatial light modulators (SLMs) for optical sectioning.
  • SLMs enable arbitrary illumination patterns and rapid adaptation of sectioning strategies.

Purpose of the Study:

  • To characterize a PAM incorporating a digital micromirror device (DMD).
  • To evaluate axial sectioning performance and compare it with confocal microscopy for biological samples.

Main Methods:

  • Comparison of three axial sectioning strategies: line scans, dot lattice scans, and pseudo-random sequence scans.
  • Analysis of axial sectioning response using fluorescent thin films.
  • Imaging of biological specimens and comparison with confocal laser scanning microscopy.

Related Experiment Videos

Main Results:

  • Axial response showed decreased Full Width at Half Maximum (FWHM) and increased offset with smaller pattern unit cells.
  • A trade-off exists between FWHM and offset for patterns with constant throughput.
  • PAM demonstrated higher signal, dynamic range, and revealed more structures in x-z sections compared to confocal microscopy, with shorter acquisition times.

Conclusions:

  • PAMs offer a flexible and powerful alternative to traditional optical sectioning microscopy.
  • The choice of scanning strategy significantly impacts performance metrics like light throughput and sectioning strength.
  • PAMs provide advantages in speed, signal, and structural detail for biological imaging.