Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Infectious RNA transcripts from grapevine virus A cDNA clone.

N Galiakparov1, E Tanne, I Sela

  • 1Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.

Virus Genes
|December 14, 1999
PubMed
Summary

Researchers created infectious RNA transcripts from a grapevine virus A (GVA) cDNA clone. This breakthrough enables efficient study of Vitivirus genus members and their impact on grapevines.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alarming levels of multidrug-resistant tuberculosis in Ukraine: results from the first national survey.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2018
Same author

Evaluation of five protocols for DNA extraction from leaves of Malus sieversii, Vitis vinifera, and Armeniaca vulgaris.

Genetics and molecular research : GMR·2014
Same author

VDAC1-based peptides: novel pro-apoptotic agents and potential therapeutics for B-cell chronic lymphocytic leukemia.

Cell death & disease·2013
Same author

Idiopathic intracranial hypertension in the USA: the role of obesity in establishing prevalence and healthcare costs.

Obesity reviews : an official journal of the International Association for the Study of Obesity·2010
Same author

G protein coupled receptors -in silico drug discovery and design.

Current topics in medicinal chemistry·2010
Same author

IAPV, a bee-affecting virus associated with Colony Collapse Disorder can be silenced by dsRNA ingestion.

Insect molecular biology·2009

Area of Science:

  • Plant virology
  • Molecular biology
  • Agricultural science

Background:

  • Grapevine virus A (GVA) is a significant pathogen affecting grape production.
  • Understanding viral replication and infection mechanisms is crucial for developing control strategies.

Purpose of the Study:

  • To construct a full-length cDNA clone of GVA.
  • To generate infectious RNA transcripts from the GVA cDNA clone.
  • To confirm the infectivity and symptomology of the transcribed RNA in host plants.

Main Methods:

  • Construction of a full-length GVA cDNA clone under a T7 promoter.
  • In vitro transcription to produce capped RNA.
  • Inoculation of Nicotiana benthamiana and N. clevelandii plants with RNA transcripts.

Related Experiment Videos

  • Serological detection of viral proteins and electron microscopy for virion observation.

    • Main Results:

    • Infectious RNA transcripts were successfully generated from the GVA cDNA clone.
    • Inoculated plants developed symptoms indistinguishable from those caused by the parental virus.
    • Viral coat and movement proteins were detected serologically, and virions were observed via electron microscopy, confirming RNA infectivity.

    Conclusions:

    • This study reports the first infectious RNA transcripts derived from a full-length cDNA clone of a Vitivirus member.
    • The developed method provides a powerful tool for studying GVA and other vitiviruses, facilitating research into their biology and pathogenesis.