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Influence of peroperative lavage solutions on peritoneal defence mechanisms in vitro
M van Westreenen1, F J Mul, A Pronk
1Department of Surgery, Diakonessen Hospital, Amsterdam, The Netherlands.
Objective:
To find out the in vitro reaction of mesothelial cells and polymorphonuclear leucocytes (PMN) to incubation with seven commonly-used lavage solutions.
Design:
Experimental study.
Setting:
Laboratories, The Netherlands.
Material:
Cultured human peritoneal mesothelial cells and isolated PMN.
Intervention:
Incubation of cells with clinically used lavage solutions (sodium chloride, Hartmann's solution, povidone-iodine, Dakin's solution, taurolidine, chlorhexidine, and hydrogen peroxide).
Main Outcome Measures:
Activation of monolayers of mesothelial cells and PMN measured by release of oxygen free radicals (chemiluminescence) and interleukin (IL)-8 concentrations and toxic effects measured by morphology, release of lactate dehydrogenase, failure of the restriction of the passage of inulin, and incorporation of propidium iodide.
Results:
All solutions activated and killed mesothelial cells and PMN to some extent; the more concentrated the solution the greater the effect on these cells.
Conclusion:
Lavage solutions both poison and stimulate mesothelial cells and neutrophils, and some solutions are more potent than others.
Insights
Common lavage solutions can harm and stimulate mesothelial cells and neutrophils. The concentration of the solution directly impacts its potency, affecting these crucial immune and barrier cells in vitro.
Area of Science:
- Cell biology
- Immunology
- Biomedical research
Background:
- Lavage solutions are frequently used in clinical settings.
- The effects of these solutions on peritoneal mesothelial cells and polymorphonuclear leucocytes (PMN) require detailed investigation.
Purpose of the Study:
- To evaluate the in vitro effects of seven common lavage solutions on human peritoneal mesothelial cells and PMN.
- To assess cellular activation and toxicity induced by these solutions.
Main Methods:
- Experimental in vitro study using cultured human peritoneal mesothelial cells and isolated PMN.
- Cells were incubated with seven clinically used lavage solutions.
- Assessed cellular responses including oxygen free radical release, IL-8 concentrations, and various toxicity markers.
Main Results:
- All tested lavage solutions demonstrated both stimulatory and toxic effects on mesothelial cells and PMN.
- A dose-dependent relationship was observed: higher concentrations of solutions resulted in greater cellular damage and activation.
- Variability in potency among the different lavage solutions was evident.
Conclusions:
- Lavage solutions possess a dual action, capable of both activating and damaging mesothelial cells and neutrophils.
- The choice and concentration of lavage solutions are critical factors influencing their impact on cellular integrity and function.
- Further research into optimizing lavage solutions for clinical use is warranted.