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Detection of soluble alpha1 integrin in human serum
1Department of Medicine, Chaim Sheba Medical Center, Sackler School of Medicine, Israel.
The Journal of Laboratory and Clinical Medicine
|December 14, 1999
Summary
A new enzyme-linked immunosorbent assay (ELISA) detects soluble alpha1beta1 integrins (salpha1) in human serum. This assay found alpha1beta1 integrins in healthy individuals and patients with connective tissue or liver diseases.
Area of Science:
- Biochemistry
- Immunology
- Clinical Diagnostics
Background:
- Soluble alpha1beta1 integrins (salpha1) are implicated in various biological processes.
- Quantifying salpha1 in human serum is crucial for understanding its role in health and disease.
Purpose of the Study:
- To develop and validate an enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of soluble alpha1beta1 integrins (salpha1) in human serum.
Main Methods:
- Developed a sandwich ELISA using anti-alpha1 integrin monoclonal antibodies (mAbs) TS2/7 (capture) and 1B3.1 (detection).
- Utilized human placental extract (PE) as a positive control and validated assay specificity against albumin and collagen.
- Assayed serum samples from normal donors and patients with connective tissue diseases (CTD) and liver diseases (LD).
Main Results:
- The assay demonstrated specificity for salpha1, with optimal binding achieved using immobilized mAb TS2/7 and PE.
- A direct correlation was observed between specific optical density (OD) and PE concentration.
- Elevated salpha1 levels (>3.4 ng/mL) were detected in serum samples from normal individuals (5/8), CTD patients (3/8), and LD patients (6/9), with low intra-assay variance (<5.7%).
Conclusions:
- This study presents the first validated ELISA for quantifying salpha1 in human serum.
- The findings indicate the presence of soluble alpha1beta1 integrins in both healthy and diseased human serum, suggesting potential roles in various physiological and pathological conditions.