Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Disposable optical sensor chip for medical diagnostics: new ways in bioanalysis.

K Schult1, A Katerkamp, D Trau

  • 1Institut für Chemo- und Biosensorik, Münster, Germany.

Analytical Chemistry
|December 22, 1999
PubMed
Summary

This novel optical sensor system enables rapid medical diagnostics at the point-of-care. It accurately detects biomarkers like human chorionic gonadotropin (hCG) and theophylline without requiring washing steps.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical characteristics and BGA-optimized pretest probability of pulmonary embolism in the elderly.

Medizinische Klinik, Intensivmedizin und Notfallmedizin·2025
Same author

Validation of the standardization framework SSTR-RADS 1.0 for neuroendocrine tumors using the novel SSTR‑targeting peptide [<sup>18</sup>F]SiTATE.

European radiology·2024
Same author

Intranasal orexin A modulates sympathetic vascular tone: a pilot study in healthy male humans.

Journal of neurophysiology·2022
Same author

Intranasal oxytocin has sympathoexcitatory effects on vascular tone in healthy males.

American journal of physiology. Regulatory, integrative and comparative physiology·2020
Same author

Oat1/3 restoration protects against renal damage after ischemic AKI.

American journal of physiology. Renal physiology·2014
Same author

Proton-stimulated opening of stomata in relation to chloride uptake by guard cells.

Planta·2014

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Medical Diagnostics

Background:

  • Point-of-care diagnostics require rapid, accurate, and user-friendly analytical systems.
  • Traditional immunoassays often involve complex procedures like washing and separation steps, limiting their point-of-care applicability.

Purpose of the Study:

  • To describe a novel optical sensor system designed for medical point-of-care diagnostics.
  • To demonstrate the system's versatility for various immunochemical assay formats.
  • To validate the system's performance for detecting specific biomarkers in human serum.

Main Methods:

  • Development of a disposable sensor chip with a capillary aperture and a ground plate for biocomponent immobilization.
  • Utilizing evanescent field generation via total internal reflection of a laser beam for fluorophor marker excitation.

Related Experiment Videos

  • Detection of fluorescence using a photomultiplier tube, eliminating the need for washing or separation steps.

    • Main Results:

    • Successful determination of human chorionic gonadotropin (hCG) in serum with a detection limit of 1 ng/mL and high recovery rates.
    • Demonstrated feasibility for competitive-type immunoassays, exemplified by the detection of theophylline in serum with a linear calibration curve.
    • Achieved low standard deviation (5.6%) in repeated measurements for hCG detection.

    Conclusions:

    • The described optical sensor system is highly suitable for point-of-care diagnostics due to its efficiency and simplicity.
    • The system's ability to perform various immunochemical assays without washing steps offers significant advantages for clinical applications.
    • The demonstrated detection of hCG and theophylline validates the system's potential for broad diagnostic utility.