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A procedure for the preparation of GM3 ganglioside from GM1-lactone
L Mauri1, R Casellato, G Kirschner
1Study Center for the Functional Biochemistry of Brain Lipids, Department of Medical Chemistry and Biochemistry - LITA-Segrate, The Medical School, University of Milan, Milano, Italy.
Glycoconjugate Journal
|December 22, 1999
Abstract:
A simple procedure is described for preparing GM3 ganglioside, from a few milligrams to grams, from GM1-lactone (Sonnino et al., (1985) Glycoconjugate J 2: 343-54) [1]. The synthesis was carried out under the following optimal conditions: 30 mM GM1-lactone in 0.25 M H2SO4 in DMSO, 30 min, 70 degrees C, nitrogen atmosphere, strong stirring. The yield of GM3 was 55%. The procedure applied to milligram amounts of GD1b-dilactone gave GD3 ganglioside.