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Related Experiment Videos

A new sequence-specific endonuclease from Streptococcus faecalis subsp. zymogenes.

R Wu, C T King, E Jay

    Gene
    |December 1, 1978
    PubMed
    Summary
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    A novel enzyme, SfaI, has been purified from Streptococcus faecalis. This sequence-specific endonuclease recognizes and cleaves the G-G-C-C DNA sequence.

    Area of Science:

    • Molecular Biology
    • Enzymology
    • Microbiology

    Background:

    • Restriction enzymes are crucial tools in molecular biology for DNA manipulation.
    • Identifying novel restriction enzymes expands the toolkit for genetic engineering and analysis.

    Purpose of the Study:

    • To report the discovery and partial purification of a new sequence-specific endonuclease.
    • To characterize the DNA recognition and cleavage site of the novel enzyme.

    Main Methods:

    • Partial purification of the endonuclease from Streptococcus faecalis subsp. zymogenes.
    • Determination of the specific DNA sequence recognized by the enzyme.

    Main Results:

    • A new sequence-specific endonuclease, designated SfaI, was partially purified.

    Related Experiment Videos

  • SfaI recognizes the tetranucleotide sequence 5'G-G-C-C 3' / 3' C-C-G-G 5'.

    • Conclusions:

    • SfaI is a novel restriction enzyme with a unique recognition site.
    • This enzyme has potential applications in molecular biology research and biotechnology.