Increased myofibroblast contractile sensitivity in paraquat pretreated rat lung tissue

In numerous tissues, contractility to certain characteristic agents is associated with cells other than muscle, e.g. actin-containing so-called myofibroblasts. Such cells are present in pulmonary interstitium of several mammalian species but their contractility has only been demonstrated from the proliferation of myofibroblasts in fibrotic lung tissues. This study has been done to evaluate such responses in normal tissues in contrast with fibrotic lungs and it was necessary to take account also of smooth muscle reactivity. Distinctive agonists: mepyramine and sodium tungstate characterised myofibroblast mediated contractility, in contrast with acetylcholine and barium chloride as specific smooth muscle stimulants. Histology and immunohistochemistry were used to evaluate the pharmacological results more precisely.