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Related Experiment Videos

Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms.

M A Zeiger1, R C Smallridge, D P Clark

  • 1Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287-8611, USA.

Surgery
|December 22, 1999
PubMed
Summary

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Detecting human telomerase reverse transcriptase (hTERT) gene expression in fine-needle aspiration (FNA) samples shows promise for diagnosing thyroid nodules. This method can help distinguish between benign and malignant thyroid lesions, potentially improving patient surgical management.

Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Endocrinology

Background:

  • Fine-needle aspiration (FNA) has limitations in definitively diagnosing thyroid carcinoma.
  • A significant percentage of patients with suspicious thyroid lesions require surgery due to inconclusive FNA results.
  • Human telomerase reverse transcriptase (hTERT) has emerged as a potential malignancy marker.

Purpose of the Study:

  • To evaluate the utility of hTERT gene expression as an adjunct diagnostic marker in thyroid fine-needle aspiration (FNA) samples.
  • To determine if hTERT expression can improve the differential diagnosis of thyroid nodules.
  • To assess the potential impact of hTERT detection on surgical management decisions.

Main Methods:

  • Collected 24 FNA samples from thyroid nodules with suspected malignancy.

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  • Extracted RNA and analyzed hTERT gene expression using reverse transcription-polymerase chain reaction (RT-PCR).
  • Performed cytologic and histologic examinations for comparison.

    • Main Results:

    • hTERT was detected in FNA samples of most thyroid carcinomas (follicular, Hürthle cell, papillary).
    • One Hürthle cell adenoma was positive for hTERT; follicular adenomas and hyperplastic nodules were negative.
    • The positive and negative predictive values for hTERT detection were 93% and 90%, respectively.

    Conclusions:

    • hTERT gene expression in thyroid FNA samples is a promising marker for differentiating benign from malignant thyroid lesions.
    • The application of hTERT detection could significantly alter the surgical management of patients with thyroid nodules.
    • This molecular marker offers improved diagnostic accuracy for suspicious thyroid findings.