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Colchicine inhibits isoflurane-induced preconditioning.
M S Ismaeil1, I Tkachenko, R F Hickey
1Department of Anesthesia, University of California-San Francisco, USA.
Anesthesiology
|December 22, 1999
Summary
Isoflurane protects the heart during ischemia and reperfusion, but this effect depends on an intact microtubular cytoskeleton. Colchicine, a microtubule-disrupting agent, abolished isoflurane
Area of Science:
- Cardiology
- Anesthesiology
- Cell Biology
Background:
- Isoflurane provides cardioprotection against myocardial ischemia-reperfusion injury.
- This protective effect mimics ischemic preconditioning.
- The role of the cytoskeleton in this process was investigated.
Purpose of the Study:
- To determine if an intact cytoskeleton is essential for isoflurane-induced cardioprotection.
- To assess the effect of colchicine, a microtubule depolymerizing agent, on isoflurane preconditioning.
Main Methods:
- A rabbit model of myocardial ischemia-reperfusion was used.
- Animals were divided into four groups: control, isoflurane preconditioning, isoflurane plus colchicine, and colchicine alone.
- Myocardial infarct size and area at risk were measured.
Main Results:
- Isoflurane preconditioning significantly reduced infarct size.
- Pretreatment with colchicine abolished the infarct-size-reducing effect of isoflurane.
- Colchicine alone did not affect infarct size.
Conclusions:
- An intact microtubular cytoskeleton is critical for the cardioprotective effects of isoflurane.
- Volatile anesthetic-induced preconditioning is dependent on cytoskeletal integrity.