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Monocytes confer CD14 antigenicity on activated lymphocytes.
Z G Fridlender1, R Rabinowitz, M Schlesinger
1Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
Human Immunology
|December 22, 1999
Summary
Activation of lymphocytes with phorbol 12-myristate 13-acetate (PMA) induces CD14 expression on lymphocytes, particularly when monocytes are present. This suggests monocytes may transfer CD14 molecules to lymphocytes.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD14 is a monocyte/macrophage surface marker.
- Lymphocytes typically do not express CD14.
Purpose of the Study:
- To investigate the expression of CD14 on lymphocytes.
- To determine the role of monocytes in CD14 expression on lymphocytes.
Main Methods:
- Human peripheral blood mononuclear cells (PBMC) were cultured with various stimuli.
- Flow cytometry was used to detect CD14 expression on lymphocytes.
- Monocytes and lymphocytes were separated using Millipore filters.
Main Results:
- PMA stimulation induced CD14 expression on up to 30% of lymphocytes.
- CD14 expression on lymphocytes was dependent on the presence of monocytes.
- Monocytes lost CD14 reactivity after PMA exposure.
- Soluble CD14 levels decreased in PMA-stimulated PBMC cultures.
Conclusions:
- Lymphocyte activation in the presence of monocytes leads to CD14 expression on lymphocytes.
- CD14 expression on lymphocytes may occur via transfer from monocytes.