Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis of gene expression in single cells.

T C Freeman1, K Lee, P J Richardson

  • 1Gene Expression Group, Wellcome Trust Genome Campus, The Sanger Centre, Hinxton, CB10 1SA, UK.

Current Opinion in Biotechnology
|December 22, 1999
PubMed
Summary

Studying gene expression in single cells offers insights into cell physiology. Advances in mRNA amplification may soon enable comprehensive gene expression analysis using DNA microarrays for individual cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of two transcription activation elements in the promoter of the developmentally regulated con-10 gene of Neurospora crassa.

Fungal genetics and biology : FG & B·1998
Same author

Tissue-specific repression of starvation and stress responses of the Neurospora crassa con-10 gene is mediated by RCO1.

Fungal genetics and biology : FG & B·1998
Same author

Structural modification of an orally active thrombin inhibitor, LB30057: replacement of the D-pocket-binding naphthyl moiety.

Bioorganic & medicinal chemistry·1998
Same author

Effects of intravenous immunoglobulin on plasma interleukin-10 levels in Kawasaki disease.

Immunology letters·1998
Same author

Randomized trial of pamidronate in patients with thyroid cancer: bone density is not reduced by suppressive doses of thyroxine, but is increased by cyclic intravenous pamidronate.

The Journal of clinical endocrinology and metabolism·1998
Same author

Isolation of MOAT-B, a widely expressed multidrug resistance-associated protein/canalicular multispecific organic anion transporter-related transporter.

Cancer research·1998

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Cellular structure and function depend on gene expression.
  • Analyzing gene usage at the single-cell level is crucial for understanding cell physiology.
  • Challenges in single-cell analysis include sample collection, size, and assay sensitivity.

Purpose of the Study:

  • To highlight the importance of single-cell gene expression analysis.
  • To discuss advancements in cellular material collection and single-cell expression analysis.
  • To introduce the potential of mRNA amplification for future single-cell studies.

Main Methods:

  • Review of current techniques for cellular material collection.
  • Evaluation of methods for single-cell gene expression analysis.

Related Experiment Videos

  • Discussion of emerging mRNA amplification technologies.

    • Main Results:

    • Progress has been made in cellular material collection for single-cell studies.
    • Single-cell gene expression analysis techniques have improved in performance.
    • Global mRNA amplification shows promise for future analyses.

    Conclusions:

    • Single-cell gene expression analysis provides valuable physiological insights.
    • Technological advancements are overcoming previous limitations in sample handling and sensitivity.
    • Global mRNA amplification is poised to enhance the scope of single-cell expression profiling on DNA microarrays.