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Related Experiment Videos

Yeast autonomously replicating sequence binding factor is involved in nucleotide excision repair.

S H Reed1, M Akiyama, B Stillman

  • 1Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA.

Genes & Development
|December 22, 1999
PubMed
Summary

Autonomously replicating sequence binding factor 1 (ABF1) is a novel component of the yeast DNA repair complex. ABF1 plays a direct role in nucleotide excision repair (NER), impacting DNA replication and UV radiation sensitivity.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Nucleotide excision repair (NER) is a crucial DNA repair pathway in yeast, involving a large protein complex.
  • A previously identified subcomplex contains the yeast Rad7 and Rad16 proteins.

Purpose of the Study:

  • To identify novel components of the yeast NER pathway.
  • To investigate the role of autonomously replicating sequence binding factor 1 (ABF1) in NER.

Main Methods:

  • Biochemical analysis to identify components of the Rad7/Rad16 subcomplex.
  • In vitro assays to assess the direct role of ABF1 in NER.
  • Analysis of temperature-sensitive abf1 mutant strains for DNA repair defects and UV sensitivity.

Main Results:

Related Experiment Videos

  • Autonomously replicating sequence binding factor 1 (ABF1) was identified as a component of the Rad7/Rad16 NER subcomplex.
  • ABF1 demonstrated a direct role in NER in vitro.
  • Specific abf1 mutant strains showed defects in photoproduct removal by NER and increased sensitivity to UV radiation.

Conclusions:

  • ABF1 plays a novel and unexpected role in yeast nucleotide excision repair.
  • The findings link ABF1's functions in DNA replication and gene silencing to its role in DNA repair.