Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In situ SR function in postinfarction myocytes.

X Q Zhang1, Y C Ng, R L Moore

  • 1Department of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, Pennsylvania 17033, USA.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|December 22, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trends in Faecal Calprotectin Levels During Pregnancy in Non-IBD Patients.

Irish medical journal·2023
Same author

Exploration of Factors Associated with Intention, Initiation and Duration of Breastfeeding.

Irish medical journal·2022
Same author

Response to "clarification of enrolled subjects in tolvaptan HF trials".

Clinical pharmacology and therapeutics·2014
Same author

Exploring the molecular mechanism of acute heat stress exposure in broiler chickens using gene expression profiling.

Gene·2014
Same author

Survivability and molecular variation in Vibrio cholerae from epidemic sites in China.

Epidemiology and infection·2014
Same author

Morphological and genetic characteristics of hybrid combinations of Dactylis glomerata.

Genetics and molecular research : GMR·2014
Same journal

Thoroughbred horses susceptible to Recurrent Exertional Rhabdomyolysis have elevated skeletal muscle mitochondrial capacities.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Change in Neutrophil-to-Lymphocyte Ratio after acute and chronic exercise: A Systematic Review and Meta-Analysis.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Ankylosing spondylitis and muscle sympathetic nerve activity: a case study.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Intracranial vasomotor and blood flow responses to light intensity aerobic exercise in young adults: a 4D flow MRI study.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Comparative assessments of the COSMED adaptive mixing chamber vs. breath-by-breath methods for oxygen uptake measurements in recreationally active adults.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Can we assess exercise metabolism from skin? Metabolomic profiles in skin dialysate collected during exercise.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
See all related articles

Myocardial infarction (MI) in rats reduces sarcoplasmic reticulum (SR) Ca(2+) content due to decreased SR Ca(2+)-ATPase activity, not increased SR Ca(2+) leak. This impacts cardiac myocyte function after heart attack.

Area of Science:

  • Cardiovascular Physiology
  • Cardiac Excitation-Contraction Coupling
  • Molecular Cardiology

Background:

  • Myocardial infarction (MI) leads to reduced intracellular Ca(2+) ([Ca(2+)](i)) and sarcoplasmic reticulum (SR) Ca(2+) content in cardiac myocytes.
  • Previous studies indicate normal L-type Ca(2+) channel activity but depressed reverse Na(+)/Ca(2+) exchange in MI myocytes.

Purpose of the Study:

  • To investigate the mechanisms responsible for reduced SR Ca(2+) content in cardiac myocytes following myocardial infarction.
  • To differentiate between impaired SR Ca(2+) uptake and enhanced SR Ca(2+) leak as causes of diminished SR Ca(2+) stores.

Main Methods:

  • Intact cardiac myocytes from sham and MI rats were used to measure SR Ca(2+) uptake and leak in situ.
  • SR Ca(2+) uptake was assessed by measuring the half-time (t(1/2)) of intracellular Ca(2+) transient decline after caffeine application.

Related Experiment Videos

  • SR Ca(2+) leak was quantified using a novel electrophysiological technique.

    • Main Results:

    • MI myocytes exhibited significantly lower systolic [Ca(2+)](i) and a prolonged t(1/2) of [Ca(2+)](i) decline, indicating reduced SR Ca(2+) uptake.
    • A 19% prolongation in t(1/2) correlated with a 23% decrease in SR Ca(2+)-ATPase expression in MI myocytes.
    • No significant difference in SR Ca(2+) leak rate was observed between sham and MI myocytes.

    Conclusions:

    • Reduced SR Ca(2+) content in MI myocytes is primarily caused by impaired SR Ca(2+) uptake, specifically due to decreased SR Ca(2+)-ATPase expression.
    • Enhanced SR Ca(2+) leak does not contribute to the diminished SR Ca(2+) stores after myocardial infarction.