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Related Experiment Videos

Pulsatile protein release from a laminated device comprising polyanhydrides and pH-sensitive complexes.

H L Jiang1, K J Zhu

  • 1Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, PR China.

International Journal of Pharmaceutics
|December 22, 1999
PubMed
Summary

This study introduces a novel device for pulsatile protein delivery using pH-sensitive complexes and degrading polyanhydrides. The system enables tunable protein release, offering a new approach for controlled therapeutic delivery.

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Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Polymer Chemistry

Background:

  • Controlled protein delivery remains a challenge in therapeutics.
  • Existing systems often lack pulsatile release capabilities.
  • Polyanhydrides and pH-sensitive complexes offer potential for tunable drug release.

Purpose of the Study:

  • To design and synthesize a novel laminated device for pulsatile protein delivery.
  • To investigate the role of polyanhydrides and pH-sensitive complexes in controlling protein release kinetics.
  • To evaluate the tunability of lag time and pulse duration for therapeutic applications.

Main Methods:

  • Synthesis of poly(sebacic anhydride)-b-polyethylene glycol (PSA-b-PEG) and poly(trimellitylimidoglycine-co-sebacic anhydride)-b-polyethylene glycol (P(TMA-gly-co-SA)-b-PEG) as isolating layers.

Related Experiment Videos

  • Incorporation of poly(methacrylic acid)/polyethoxazoline (PMAA/PEOx) complexes for protein loading.
  • Fabrication of a laminated device for protein delivery.
  • In vitro assessment of protein release profiles (Myoglobin, Bovine Serum Albumin) under varying pH conditions.

    • Main Results:

    • The device successfully achieved pulsatile protein release.
    • Lag time before release correlated with polyanhydride erosion (30-165 h).
    • Pulse duration was adjustable (18.5-40 h) by varying complex mass.
    • Protein release was pH-dependent, with dissociation at pH 7.4 and retention below pH 5.0.

    Conclusions:

    • The developed device demonstrates effective pulsatile protein delivery.
    • Synergistic effects of degrading polyanhydrides, pH-sensitive complexes, and proteins enable controlled release.
    • The system offers tunable parameters for tailored therapeutic protein delivery.